Journal article
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4
JWE Lim, RA Mathias, EA Kapp, MJ Layton, MC Faux, AW Burgess, H Ji, RJ Simpson
Electrophoresis | WILEY-BLACKWELL | Published : 2012
Abstract
Mutations in the adenomatous polyposis coli (APC) tumor suppressor gene are common in both inherited and sporadic forms of colorectal cancer (CRC), and are associated with dysregulated Wnt signaling. Colon carcinoma SW480 cells restored with stable expression of wild-type APC (SW480APC cells) exhibit attenuated Wnt signaling, and reduced tumorigenicity, including increased cell adhesion. We performed a comparative proteomic analysis of exosomes isolated from SW480 and SW480APC cells to examine the effects of restored APC on exosome protein expression. A salient finding of our study was the unique expression of the Wnt antagonist Dickkopf-related protein 4 (DKK4) in SW480APC, but not parental..
View full abstractGrants
Awarded by National Health & Medical Research Council of Australia
Awarded by RD Wright Fellowship
Funding Acknowledgements
This work was supported, in part, by the National Health & Medical Research Council of Australia (program grant #487922 (R.J.S and A. W. B), a University of Melbourne Australian Postgraduate award (J.W.E.L), and an RD Wright Fellowship #164821 (M.J.L). Analysis of proteomic data described in this work was supported using the Australian Proteomics Computational Facility funded by the National Health & Medical Research Council of Australia grant #381413. This work was supported by funds from the Operational Infrastructure Support Program provided by the Victorian Government Australia. We acknowledge the Australian Cancer Research Foundation for providing funds to purchase the Orbitrap (TM) mass spectrometer.